Application of tissue engineering technology for formation of human articular cartilage in perfusion bioreactor
نویسندگان
چکیده
منابع مشابه
bovine articular cartilage decellularized matrix as a scaffold for use in cartilage tissue engineering
extracelllar matrix (ecm) materials from mammalian tissues have been broadly used for tissue engineering for animal models. while decellularization of tissues such as bladder, heart valve, knee meniscus and tendon has been performed, there are a few reports for articular cartilage. in this study we aimed to develop a technique to decellularize bovine articular cartilage as a biological construc...
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Efficient transport of regulatory molecules is one of the main requirements for directing the growth and differentiation of bone marrow stromal cells (BMSC). We have designed and utilized a packed bed bioreactor system for cultivation of murine BMSC immobilized in alginate microbeads produced by electrostatic droplet generation. Continuous medium perfusion at velocities that are physiological f...
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In this study, we first aimed at determining whether human articular chondrocytes (HAC) proliferate in aggregates in the presence of strong chondrocyte mitogens. We then investigated if the aggregated cells have an enhanced chondrogenic capacity as compared to cells cultured in monolayer. HAC from four donors were cultured in tissue culture dishes either untreated or coated with 1% agarose in t...
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BACKGROUND Dedifferentiation of chondrocytes remains a major problem for cartilage tissue engineering. Chondrocytes loss differentiated phenotype in in vitro culture that is undesired for repair strategies. The chondrocyte is surrounded by a pericellular matrix (PCM), together forming the chondron. PCM has a positive effect on the maintenance of chondrocyte phenotype during culture in compar...
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INTRODUCTION Using functional tissue engineering approaches, a number of culture systems have been developed that incorporate physiologic mechanical signals to foster tissue development (1,2). Short-term studies demonstrate that dynamic deformational loading increases matrix biosynthesis in chondrocyte-seeded agarose hydrogels (3,4). In long term studies, daily dynamic deformational loading (1H...
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ژورنال
عنوان ژورنال: Russian Journal of Transplantology and Artificial Organs
سال: 2017
ISSN: 2412-6160,1995-1191
DOI: 10.15825/1995-1191-2017-3-81-92